Sergey Malchenko, MD, PhD
Research Assistant Professor
Cancer Biology and Pharmacology
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1 Illini Drive, Peoria, IL, 61605
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About
Lab
The main focus of the Soares & Malchenko laboratory is to study mechanisms of mitochondrial retrograde signaling. The communication between Neural Stem Cells (NSCs) and surrounding astrocytes is essential for the homeostasis of NSC niche. Intercellular mitochondrial transfer, a unique communication system that utilizes formation of tunneling nanotubes for targeted mitochondrial transfer between donor and recipient cells, has recently being identified in a wide range of cell types. Intercellular mitochondrial transfer has also been observed between different types of Cancer Stem cells (CSCs) and the neighboring cells, including brain CSCs and astrocytes. The CSC mitochondrial transfer significantly enhance overall tumor progression by reprogramming the neighboring cells. Despite the urgent need to investigate this newly identified phenomenon, the mitochondrial transfer in CNS remains largely uncharacterized.
As the exact molecular pathways of the NSC and/or brain CSC mitochondria signaling that impact transcriptome of the recipient astrocytes remain to be decoded, we created a reproducible model utilizing large quantities of radial glia cells, brain tumor initiating cells, and the astrocytes that were generated from the same induced pluripotent stem cells (iPSC). Currently we are conducting different types of experimentations with this model, which involve epigenetics and metabolomics studies, to give us new insight into the mechanisms of mitochondrial retrograde signaling.
One of the goals in our lab is to provide a venue for communication and education in the field of stem cell experimental models of human diseases to promote interdisciplinary collaboration.
Positions and Appointments
- 2015-Present: Research Assistant Professor, University of Illinois College of Medicine at Peoria, IL
- 2014-2015: Director, Human Disease Models Core. Children’s Memorial Research Center, Chicago, IL
- 2005-2015: Research Assistant Professor, Northwestern University’s Feinberg School of Medicine, Chicago, IL
- 1998-2005: Assistant Research Scientist, University of Iowa, Iowa City, IA
- 1996-1998: Research Scientist, Danish Institute of Agricultural Science, Tjele, Denmark
- 1996: Guest Researcher, Danish Institute of Agricultural Science, Tjele, Denmark
- 1995: Guest Researcher, Royal Veterinary and Agricultural University, Copenhagen, Denmark
- 1994: Guest Researcher, Danish Institute of Animal Science, Foulum, Denmark
- 1991-1994: Research Fellow, Institute of Cytology and Genetics, Novosibirsk, Russia
- 1987-1988: Research Fellow, Institute of Molecular Genetics, Moscow, Russia
Contribution to Science
Collaboration for Institute of Cytology and Genetics (1994)
In 1994 I was invited to Royal Veterinary and Agricultural University, Copenhagen, Denmark and to Danish Institute of Animal Science to participate in the collaborative project initiated by Institute of Cytology and Genetics, Novosibirsk, Russia. The results of the project were published in several international scientific journals and proceedings, some of which are listed here on this profile.
Subcontract from Northwestern University’s NCI Designated Robert H Lurie Comprehensive Cancer Center (06/01/13–05/31/14).
This seed grant supported collaboration with Dr. Vinayak Dravid, to develop magnetic nanoparticles that can specifically target radial glial cells for MRI-based in vivo imaging of neural stem cells in the brain.
An exciting collaboration has also been established with Dr. Herbert Meltzer, Professor of Psychiatry & Behavioral Sciences at the Feinberg School of Medicine, Dr. Richard J. Miller, Alfred Newton Richards Professor of Pharmacology at the Feinberg School of Medicine, and with Dr. Hitoshi Hashimoto, Professor of Molecular Neuropharmacology in the Graduate School of Pharmaceutical Sciences at the Molecular Research Center for Children’s Mental Development, United Graduate School of Child Development, Osaka University in Japan. This collaborative project is aimed at uncovering the molecular basis for treatment resistance in schizophrenia. We will exploit the methodology to generate radial glial cells to derive different types of neurons from patients that have been classified according to their treatment response profiles.
In collaboration with Dr. Rishi Lulla, from the Department of Pediatrics at the Feinberg School of Medicine, we generated a collection of iPSC and radial glial cell lines from brain tumor patients with the ultimate goal of utilizing them to create mouse models of pediatric brain tumors.
Sergey Malchenko, Simone Treiger Sredni, Atsushi Kasai, Kazuki Nagayasu, Kaoru Seiriki, Jianping Xie, Naira Margaryan, Hitoshi Hashimoto, Rishi Lulla, Lauren Pachman, Herbert Y. Meltzer, Mary J.C. Hendrix and Marcelo B. Soares. 2015. A Mouse Model of Radial Glial Cell-Derived Primitive Neuroectodermal Tumors. PLOS ONE Mar 31;10(3):e0121707.
Also, in collaboration with Dr. Lauren Pachman from the Department of Pediatrics, Division of Pediatric Rheumatology, Northwestern University, Feinberg School of Medicine, we are working toward the generation of a unique collection of iPSC lines from JDM patients, which will include identical twins. The development of muscle cells from these monozygotic twins iPSC lines would be a novel and very exciting approach to understanding the disease pathophysiology.
Other work (07/01/14–06/30/16)
- Parent R21: Identical Twins Discordant for Juvenile Dermatomyositis: iPSC-Myogenic Cells.
Selected Publications
Boyineni, J.; Wood, J.M.; Ravindra, A.; Boley, E.; Donohue, S.E.; Soares, M.B.; Malchenko, S. Prospective Approach to Deciphering the Impact of Intercellular Mitochondrial Transfer from Human Neural Stem Cells and Brain Tumor-Initiating Cells to Neighboring Astrocytes. Cells 2024, 13, 204. https://doi.org/10.3390/cells13030204
Koroleva, I. V., Malchenko, S. N., Shukri, N. M., Ivanova, I. V., Kuznetsov, N. S., Zhdanova, N. S., Bendixen, C. 1998. Assignment of five porcine genes by pig-mink cell hybrids to pig chromosomes 2, 5, 8, 12. Mammalian Genome 9, 913-914.
Brusgaard K, Shukri N, Malchenko SN, Lohi O, Christensen K, Kruse T. 1998. Three polymorphic mink, Mustela vison, dinucleotide repeats. Anim. Genet. Apr; 29(2):153.
Malchenko, S.N., Golovin, S.J., Matveeva, N.M., Brusgaard, K., Serov, O.L. 1994. Cloning, nucleotide sequence and chromosomal localization of Growth Hormone of American mink (Mustela vison). Proceedings of 11th European Colloquium on Cytogenetics of Domestic Animals, Royal Veterinary and Agricultural University, Copenhagen, Denmark. August 2-5. Pp.140-144.
Koroleva, I.V., Malchenko S.N., Brusgaard, K., Zakian, S. M. 1996. Chromosome Localization of the Gene for g-chain Immunoglobulin in Silver Fox (Vulpes fulvus).The 5th Workshop of the Nordic Genome Initiative, University Hospital of Iceland. June 14-17, p. 76.
Malchenko, S. N., Koroleva, I. V., Brusgaard, K., Matveeva, N. M., Serov, O. L. 1996. Chromosome Localization of the Gene for Ornithine Transcarbamylase in American mink (Mustela vison).The 5th Workshop of the Nordic Genome Initiative, University Hospital of Iceland. June 14-17, p. 82.
Brusgaard, K., Malchenko, S.N., Shukri, N.M., Lohi, O.1996. Restriction Fragment Length Polymorphism at the MHC Class11 locus of EcoR1 digested genomic DNA in different Danish breeds of domesticated American mink (Mustela vison L.) using porcine DQa as a probe. The 5th Workshop of the Nordic Genome Initiative, University Hospital of Iceland. June 14-17, p. 64.
Koroleva I.V., Malchenko S.N., Brusgaard K., Khlebodarova T.M., Rubtsov N.B., Zakian S.M. 1996. Chromosome localization of the gene for somatostatin peptide in silver fox (Vulpes fulvus). The 6th International Congress in Fur Animal Production. Warszawa, Poland. August 21-23. Scientifur, vol.20, no.4. p. 359.
Malchenko S.N., Koroleva I.V., Brusgaard K., Khlebodarova T.M., Rubtsov N.B., Zakian S.M. 1996. Chromosome localization of the gene for growth hormone in silver fox (Vulpes fulvus). The 6th International Congress in Fur Animal Production. Warszawa, Poland. August 21-23. Scientifur, vol.20, no.4. p.358.
Brusgaard K., Malchenko S.N., Lohi O., Christensen, K. 1996. Characterization of DNA microsatellite containing cosmid mapping to American mink (Mustela vison) chromosome Y by in situ hybridization. The 6th International Congress in Fur Animal Production. Warszawa, Poland. August 21-23. Scientifur, vol.20, no.4. p.357.
Education
1987 M.D., Altay State Medical Institute, Barnaul, Russia
1996 Ph.D. with distinction, Institute of Cytology and Genetics, Novosibirsk, Russia
Professional Memberships
- 2014-Present: Member, Editorial Board, Archives of Stem Cell Research Journal
- 2012-Present: Member, Robert H. Lurie Comprehensive Cancer Center
- 2005-Present: Member, American Association for Cancer Research
- 1994-1999: Member, Danish Molecular Biology Society
- 1991-1999: Member, Russian Society of Genetics