Publications

Contribution to Science

In 1994 I was invited to Royal Veterinary and Agricultural University, Copenhagen, Denmark and to Danish Institute of Animal Science to participate in the collaborative project initiated by Institute of Cytology and Genetics, Novosibirsk, Russia. The results of the project were published in several international scientific journals and proceedings:

Khlebodarova, T.M., Malchenko, S.N., Matveeva, N.M., Pack, S.D., Sokolova, O.V., Alabiev, B.Y., Belousov, E.S., Peremislov, V.V., Nayakchin, A.M., Brusgaard, K., Serov, O.L. 1995. Chromosomal and regional localization of the loci for IGKC, IGGC, ALDB, HOXB, GDT, and PRNP in the American mink (Mustela vison): comparison with man and mouse. Mammalian Genome. 6, 10, 705-109.

Malchenko S.N., Koroleva I.V., Brusgaard K., Khlebodarova T.M., Rubtsov N.B., Zakian S.M. 1996. Chromosome localization of the gene for growth hormone in silver fox (Vulpes fulvus). Applied Science Reports, Progress in fur animal science, 27, 51-55.

Koroleva I.V., Malchenko S.N., Brusgaard K., Khlebodarova T.M., Rubtsov N.B., Zakian S.M. 1996. Chromosome localization of the gene for somatostatin peptide in silver fox (Vulpes fulvus). Applied Science Reports, Progress in fur animal science, 27, 45-49.

Malchenko, S.N., Koroleva, I.V., Brusgaard, K., Matyakhina, L.D., Colonin, M.G., Pack, S.D., Searle, J.B., Borodin, P.M., Serov, O.L. and Bendixen, C. 1996. Chromosome localization of the gene for growth hormone in the common shrew (Sorex araneus). Heredias 125, 243-245.

Koroleva, I.V., Malchenko, S.N., Khlebodarova, T.M., Brusgaard, K., Zakian. S. M. 1996. Chromosomal localization of the loci GH, SST, OTC, and PRNP in the silver fox (vulpis fulvus). Mammalian Genome 7, 860-862.

Matyakhina, L. D., Koroleva, I. V., Malchenko, S. N., Bendixen, C., Cheryaukene, O. V., Pack, S. D., Borodin, P. M., Serov, O. L. and Searle J. B. 1997. Chromosome location of sixteen genes in the common shrew, Sorex araneus L. (Mammalia, Insectivora). Cytogenet. Cell Genet. 77, 201-204.

Koroleva, I. V., Malchenko, S. N., Shukri, N. M., Ivanova, I. V., Kuznetsov, N. S., Zhdanova, N. S., Bendixen, C. 1998. Assignment of five porcine genes by pig-mink cell hybrids to pig chromosomes 2, 5, 8, 12. Mammalian Genome 9, 913-914.

Brusgaard K, Malchenko SN, Christensen K, Lohi O, Kruse T. 1998. A polymorphic mink (Mustela vison) dinucleotide repeat. Anim. Genet. Dec; 29(6):467.

Brusgaard K, Shukri N, Malchenko SN, Lohi O, Christensen K, Kruse T. 1998. Three polymorphic mink, Mustela vison, dinucleotide repeats. Anim. Genet. Apr; 29(2):153.

Malchenko, S.N., Golovin, S.J., Matveeva, N.M., Brusgaard, K., Serov, O.L. 1994. Cloning, nucleotide sequence and chromosomal localization of Growth Hormone of American mink (Mustela vison). Proceedings of 11th European Colloquium on Cytogenetics of Domestic Animals, Royal Veterinary and Agricultural University, Copenhagen, Denmark. August 2-5. Pp.140-144.

Koroleva, I.V., Malchenko S.N., Brusgaard, K., Zakian, S. M. 1996. Chromosome Localization of the Gene for g-chain Immunoglobulin in Silver Fox (Vulpes fulvus).The 5th Workshop of the Nordic Genome Initiative, University Hospital of Iceland. June 14-17, p. 76.

Malchenko, S. N., Koroleva, I. V., Brusgaard, K., Matveeva, N. M., Serov, O. L. 1996. Chromosome Localization of the Gene for Ornithine Transcarbamylase in American mink (Mustela vison).The 5th Workshop of the Nordic Genome Initiative, University Hospital of Iceland. June 14-17, p. 82.

Brusgaard, K., Malchenko, S.N., Shukri, N.M., Lohi, O.1996. Restriction Fragment Length Polymorphism at the MHC Class11 locus of EcoR1 digested genomic DNA in different Danish breeds of domesticated American mink (Mustela vison L.) using porcine DQa as a probe. The 5th Workshop of the Nordic Genome Initiative, University Hospital of Iceland. June 14-17, p. 64.

Koroleva I.V., Malchenko S.N., Brusgaard K., Khlebodarova T.M., Rubtsov N.B., Zakian S.M. 1996. Chromosome localization of the gene for somatostatin peptide in silver fox (Vulpes fulvus). The 6th International Congress in Fur Animal Production. Warszawa, Poland. August 21-23. Scientifur, vol.20, no.4. p. 359.

Malchenko S.N., Koroleva I.V., Brusgaard K., Khlebodarova T.M., Rubtsov N.B., Zakian S.M. 1996. Chromosome localization of the gene for growth hormone in silver fox (Vulpes fulvus). The 6th International Congress in Fur Animal Production. Warszawa, Poland. August 21-23. Scientifur, vol.20, no.4. p.358.

Brusgaard K., Malchenko S.N., Lohi O., Christensen, K. 1996. Characterization of DNA microsatellite containing cosmid mapping to American mink (Mustela vison) chromosome Y by in situ hybridization. The 6th International Congress in Fur Animal Production. Warszawa, Poland. August 21-23. Scientifur, vol.20, no.4. p.357.

I.V. Koroleva, S. N. Malchenko, N. M. Shukri, N. S. Zhdanova, & C. Bendixen. 1998. Assignment of porcine genes by pig-mink cell hybrids with single pig chromosomes 2, 5, 8, 12. Proceedings of the 6th World Congress on Genetics Applied to Livestock Production. Armidale, NSW, Australia. January 11-16, vol. 23, pp. 648-651.

As an Assistant Research Scientist in Dr. Marcelo Bento Soares lab at University of Iowa, I modified the existing SAGE technology by utilizing the advanced methodology of cDNA library construction developed by Dr. Soares. As the result of this modification a panel of SAGE libraries derived from chondrosarcoma patients were submitted to NCI Cancer Genome Anatomy Project public database (the only source of chondrosarcoma SAGE data available at the time).

Malchenko S, Elisabeth Seftor, Yuri Nikolsky, Susan Hasegawa, Sean Kuo, Jeff Stevens, Stas Poyarkov, Tatiana Nikolskaya, Tamara Kucaba, Min Wang, Hakim Abdulkawy, Thomas Casavant, Jose A. Morcuende, Joseph A. Buckwalter, Raymond J. Hohl, Barry R. DeYoung, Kemp Howard Kernstine, Maria de F. Bonaldo, Mary J. Hendrix, Marcelo B. Soares. 2012. Putative Multi-functional Signature of Lung Metastases in Dedifferentiated Chondrosarcoma. Sarcoma Volume 2012 (2012), Article ID 820254.

Recently, I developed a method to derive radial glial enriched neural stem cells from hESC or iPSC lines. Radial glial cells are thought to be the progenitor cells of adult neural stem cells. The ability to generate these cells in amounts is very significant because it enables a wide range of experimentation that otherwise could not be done.

Sergey Malchenko, Jianping Xie, Maria de Fatima Bonaldo, Elio F. Vanin, Bula Bhattacharyya,Vasily Galat, William Goossens, Richard E.B. Seftor, John Crispino, Richard Miller, Martha C. Bohn, Mary J.C. Hendrix and Marcelo B. Soares.2014. Onset of rosette formation during spontaneous neural differentiation of hESC and hiPSC colonies. GENE 534 400-407.

This development created an opportunity for the establishment of new collaborations that will enable grant applications that could not have been sought before. One of such collaborations is with Dr. Vinayak Dravid from the Northwestern International Institute for Nanotechnology. We are taking advantage of our ability to derive unrestricted amounts of RG cells to experiment with nanoparticles of different compositions to attain conditions under which radial glial cell specificity may be attained. This would enable diagnostic mapping of radial glial cells in the human brain by MRI, in normal brain and in the brain of individuals affected with neurodegenerative diseases. Next, we seek to identify differences between normal and malignant radial glial cells with the goal of using nanoparticles to specifically target and kill malignant radial glial cells that would otherwise function as brain tumor stem cells.

06/1/13 – 05/31/14
Subcontract from Northwestern University’s NCI Designated Robert H Lurie Comprehensive Cancer Center.
This seed grant supported collaboration with Dr. Vinayak Dravid, to develop magnetic nanoparticles that can specifically target radial glial cells for MRI-based in vivo imaging of neural stem cells in the brain.

An exciting collaboration has also been established with Dr. Herbert Meltzer, Professor of Psychiatry & Behavioral Sciences at the Feinberg School of Medicine, Dr. Richard J. Miller, Alfred Newton Richards Professor of Pharmacology at the Feinberg School of Medicine, and with Dr. Hitoshi Hashimoto, Professor of Molecular Neuropharmacology in the Graduate School of Pharmaceutical Sciences at the Molecular Research Center for Children’s Mental Development, United Graduate School of Child Development, Osaka University in Japan. This collaborative project is aimed at uncovering the molecular basis for treatment resistance in schizophrenia. We will exploit the methodology to generate radial glial cells to derive different types of neurons from patients that have been classified according to their treatment response profiles.

In collaboration with Dr. Rishi Lulla, from the Department of Pediatrics at the Feinberg School of Medicine, we generated a collection of iPSC and radial glial cell lines from brain tumor patients with the ultimate goal of utilizing them to create mouse models of pediatric brain tumors.

Sergey Malchenko, Simone Treiger Sredni, Atsushi Kasai, Kazuki Nagayasu, Kaoru Seiriki, Jianping Xie, Naira Margaryan, Hitoshi Hashimoto, Rishi Lulla, Lauren Pachman, Herbert Y. Meltzer, Mary J.C. Hendrix and Marcelo B. Soares. 2015. A Mouse Model of Radial Glial Cell-Derived Primitive Neuroectodermal Tumors. PLOS ONE Mar 31;10(3):e0121707.

Also, in collaboration with Dr. Lauren Pachman from the Department of Pediatrics, Division of Pediatric Rheumatology, Northwestern University, Feinberg School of Medicine, we are working toward the generation of a unique collection of iPSC lines from JDM patients, which will include identical twins. The development of muscle cells from these monozygotic twins iPSC lines would be a novel and very exciting approach to understanding the disease pathophysiology.

07/01/14-06/30/16
Parent R21: Identical Twins Discordant for Juvenile Dermatomyositis: iPSC-Myogenic Cells.