Swapna Asuthkar

Special Competencies and Interests

Cell biology: Cell lines/primary cell culture, cell growth curve, angiogenesis in vitro/in vivo, cell adhesion and chemotactic migration assay, cell apoptosis and differentiation assays, immunofluorescent double/triple staining, cell sorting by FACS, transfection, cancer stem cells, protein extraction and purification.

Immuno-biology: role of Immune checkpoints the tumor-microenvironment, Enzyme linked immunosorbent assay (ELISA), ELISPOT, microscopic agglutination test (MAT), serotyping, immunochemical analysis of human serum and its fractions, immunoprecipitation, immunoelectrophoresis, immunocytochemistry, immunohistochemistry, flow cytometry, LC/MS/MS analysis.

Microbiolgy: aseptic and sterile techniques, bacterial staining, plating methods (streak, spread, pour, replica), cell transformation, enumeration and identification of bacteria, use of biological safety cabinets, media and buffer preparation, plaque assay.

Epigenetics: CpG island analysis, bisulphite modification and sequencing, MSP and USP analysis, Histone modifiers; methylases and acetylases.

Tumor biology: Animal models (intracranial, subcutaneous and tail vein injections), tumorigenicity, live imaging, animal dissection, chemo and radiotherapy, cell proliferation (clonogenic, FACS and MTT assay), apoptosis (TUNEL, apoptotic DNA fragmentation analysis and mitochondrial apoptosis detection), tumor cell adhesion, invasion, migration, angiogenesis wound repair and metastasis, Multidrug Resistance Direct Dye Efflux Assays, CSC sphere formation, Chemotaxis.

Microscopy: Light microscopy, fluorescent; confocal microscopy, transmission electron microscopy.

Protein/Molecular biology: Data mining. RNA seq analysis, DNA recombination, plasmid/retroviral plasmid construction, sub-cloning, sequencing, stable/transient transfections, site-directed mutagenesis, siRNA, PCR, RT-PCR, ChIP, in situ hybridization, DNA and RNA isolation, Southern and Northern blotting, EMSA, DNA and protein arrays, transcription factor (TF) binding site prediction, TF-TF/TF-DNA interactions, sub cellular fractionation, culture filtrate fractionation, fibrin and gelatin zymography, ubiquitination, LC/MS/MS analysis,.

miRNA methods: miRNAs and antago-miRNAs, expression profiling, quantification and target identification, 3’ UTR reporter assay, stem-loop PCR.

Exosome research: Exosome isolations, quantification and analysis.

Pathology: Histological and pathological diagnosis, techniques for sample/section preparation and H&E staining, immunohistological double/triple staining.

Biophysics: Calcium Imaging, planar lipid-bilayers.